ABSTRACT：The polyphenolic profiles by HPLC-TOF-MS of strawberry ‘San Andreas’ and blackberry ‘Black Satin’ crudeextracts (CE) were analyzed. Anthocyanin-enriched fractions (AEFs) and proanthocyanidin-enriched fractions(PEFs) were prepared, and all samples were probed for in vitro anti-inflammatory and wound healing effects in aLPS-stimulated RAW 264.7 macrophage model and in a skin fibroblast migration and proliferation assay, respectively.Blackberry samples exhibited higher ROS reduction than strawberry's (up to 50% ROS suppression).Berries CEs exhibited 20% inhibition in Cox-2 gene expression, while AEFs and PEFs were inactive at the sameconcentration. Strawberry AEF and PEF were more active against IL-1β and IL-6 gene expressions than thesimilar fractions from blackberry, where PEF was more active than AEF (75% suppression by strawberry PEF).Moreover, berry PEFs were the active polyphenol fraction against iNOS gene expression (50% and 65% gensuppression by strawberry and blackberry PEF, respectively), mirroring results of NO synthesis suppression. The
cell migration potential of berry polyphenolics was associated with anthocyanins. AEFs showed fibroblast migrationaround 50% of that registered for the positive control. Results obtained in this work highlight the antiinflammatoryproperties of berry polyphenolics, especially due to proanthocyanidins. Moreover, promising resultswere obtained about the effects of berry anthocyanins on wound healing.

KEY WORDS: Fragaria ananassa;Rubus fruticosus;Anthocyanins;Proanthocyanidins;Cyclooxygenase-2;Interleukin-6;Skin cell migration

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Anti-inflammatory and wound healing properties of polyphenolic extracts
from strawberry and blackberry fruits

Franco Van de Veldea,b,⁎, Debora Espositoc,d, Mary H. Gracec, María E. Pirovania, Mary A. Lilac

a Instituto de Tecnología de Alimentos, Facultad de Ingeniería Química, Universidad Nacional del Litoral, Santiago del Estero 2829, Santa Fe, 3000, Argentina
b Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Santiago del Estero 2829, Santa Fe, Argentina
c Plants for Human Health Institute, Food Bioprocessing and Nutrition Sciences Department, North Carolina State University, North Carolina Research Campus, 600
Laureate Way, Kannapolis, NC 28081, United States
d Department of Animal Science, North Carolina State University, 101 Polk Hall, 120 Broughton Drive, Raleigh, NC 27695, United States

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