ABSTRACT：Flavonoids have been presented as potential protectors against metabolic and cognitive dysfunction. However, mechanisms underlying these ‘claims’ are not sufficiently explored. To analyse the effect of long-term supplementation with blackberry extract (BE) in a context of high-fat or standard diet, Wistar rats were divided in 4 groups (n=6) fed with standard or high-fat diet, with or without BE supplementation of 25 mg/kg body weight per day. High-fat diet significantly impaired glucose tolerance and increased: body weight, caloric ingestion, very-low-density lipoprotein, triglycerides and cholesterol. Furthermore, it was observed that high-fat diet increased dopamine content in prefrontal cortex and decreased BDNF levels both in prefrontal cortex and plasma. BE supplementation only affected part of those effects. BE slightly improved glucose metabolism and significantly decreased levels of lactate, independently of diet. BE decreased levels of BDNF and also interacted with dopaminergic system, increasing dopamine turnover in striatum, and reverting dopamine content induced by high-fat diet in prefrontal cortex. This study shows that although some particular benefits of anthocyanins supplementation some long-term effects may not be desirable and further studies are needed to optimize ingestion conditions.

KEY WORDS: anthocyanins, dopaminergic system, flavonoids, high-fat diet, neuroprotection,obesity

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Effect of chronic consumption of blackberry extract on high-fat induced obesity  in rats and its correlation with metabolic and brain outcomes

Manuela Meireles1, Luís M. Rodríguez-Alcalá2, Cláudia Marques1, Sónia Norberto13 , Joana
Freitas1, Iva Fernandes3, Nuno Mateus3, Ana Gomes2, Ana Faria1,3,4, Conceição Calhau1,5*

 Departamento de Bioquímica, Faculdade de Medicina, Universidade do Porto, Al. Prof.
 Hernâni Monteiro, 4200-319 Porto, Portugal
 Centro de Biotecnologia e Química Fina (CBQF), Escola Superior de Biotecnologia,
 Universidade Católica Portuguesa, Rua Dr. António Bernardino de Almeida, 4200-072 Porto,Portugal
 REQUIMTE, Laboratório Associado de Química Verde, Faculdade de Ciências, Universidade do Porto, 4169-009 Porto, Portugal
 Faculdade de Ciências da Nutrição e Alimentação, Universidade do Porto, Rua Dr. Roberto
 Frias, 4200-465 Porto, Portugal
 Centro de Investigação em Tecnologias e Sistemas de Informação em Saúde (CINTESIS),
 Universidade do Porto, Al. Prof. Hernâni Monteiro 4200-319 Porto, Portugal

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