Blackberry and Blueberry Anthocyanin Supplementation Counteract High-Fat-Diet-Induced Obesity by Alleviating Oxidative Stress and Inflammation and Accelerating Energy Expenditure
Many studies indicate that an anthocyanin-rich diet has beneficial effects preventing metabolic disease. In the present study, the molecular mechanism underlying the antiobesity effect of consuming blackberry anthocyanins (BLA) and blueberry anthocyanins (BBA) was investigated in high-fat-diet- (HFD-) fed C57BL/6 mice. Sixty mice were administered a low-fat diet (LFD), a HFD, or a HFD plus orlistat, and BLA or BBA in their daily food for 12 weeks. As a result, the consumption of BLA and BBA inhibited body weight gain by 40.5% and 55.4%, respectively, in HFD-fed mice. The BLA and BBA treatments markedly reduced serum and hepatic lipid levels and significantly increased hepatic superoxide dismutase and glutathione peroxidase activities. In addition, the treatments effectively increased fecal acetate and butyrate levels and significantly attenuated expression of tumor necrosis factor TNF-α, interleukin-6, and nuclear factor-kappaB genes. Moreover, gas chromatography time-of-flight mass spectroscopy results suggested that BLA and BBA significantly affected the hepatic lipid and glucose metabolic pathways, including glycerophospholipid metabolism, glutathione metabolism, and the insulin-signaling pathway. Therefore, BLA and BBA ameliorated diet-induced obesity by alleviating oxidative stress and inflammation and accelerating energy expenditure.
许多研究表明，富含花青素的饮食对预防代谢性疾病有有益的效果。本研究以高脂饮食(HFD-)喂养的C57BL/6小鼠为实验对象，探讨了黑莓花青素(BLA)和蓝莓花青素(BBA)抗肥胖作用的分子机制。60只小鼠连续12周接受低脂饮食(LFD)、高脂饮食(HFD)或高脂饮食加奥利司他，以及BLA或BBA。结果表明，在hfd喂养的小鼠中，BLA和BBA的摄入分别抑制了体重增加40.5%和55.4%。BLA和BBA处理可显著降低血清和肝脏脂质水平，显著提高肝脏超氧化物歧化酶和谷胱甘肽过氧化物酶活性。此外，处理可有效提高粪便乙酸和丁酸水平，并显著降低肿瘤坏死因子TNF-α、白细胞介素-6和核因子- kappab基因的表达。此外，气相色谱飞行时间质谱分析结果表明，BLA和BBA显著影响肝脏脂质和葡萄糖代谢途径，包括甘油磷脂代谢、谷胱甘肽代谢和胰岛素信号通路。因此，BLA和BBA通过减轻氧化应激和炎症和加速能量消耗来改善饮食诱导的肥胖。
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