热带高地黑莓酚类化合物的体外抗氧化和抗炎活性

Abstract:This study evaluates the antioxidant and anti-inflammatory activities in a polyphenol extract from blackberries.The antioxidant activity measured via oxygen radical absorbance capacity (ORAC) was higher for the blackberry extract (4339 ±144 μM TE/g) than for quercetin and ellagic acid. The blackberry phenolic compounds protected liposomes and liverhomogenates against lipid peroxidation; in both models, the antioxidant activity (IC50 = 7.0 ± 0.5 and 20.3 ± 4.2 μg/mL,respectively) was greater than that found with Trolox. The extract inhibited superoxide production by NADPH oxidase in THP-1 cells and nitrite production in J774A.1 cells stimulated with LPS+IFNγ, with nitrite production decreasing after 4 h of incubation with the extract, mainly through a strong scavenging activity. However, 24 h of treatment reduced the amount of nitrites (IC50 =45.6 ± 1.2 μg/mL) because of a down-regulation of iNOS protein expression, as demonstrated by Western blotting. The inhibitory activities found in blackberry phenols suggest a potential beneficial effect against oxidative stress and inflammatory processes.

Key words: blackberry, Rubus adenotrichos, antioxidant activity, lipid peroxidation, anion superoxide, iNOS, macrophages cell lines

†Departamento de Bioquímica, Facultad de Medicina, Universidad de Costa Rica, Código Postal 11501-2060 San José, Costa Rica‡Laboratoire d’Immunologie, Faculté de Pharmacie, Université Montpellier 1, UMR 95 QUALISUD, 34967 Montpellier Cedex 2,France§Institut Universitaire de Recherche Clinique, Université Montpellier 1, UMR 204 Nutripass, 34967 Montpellier Cedex 2, France∥Laboratoire de Biochimie, CHU Lapeyronie, Montpellier, France⊥Centro Nacional de Ciencia y Tecnología de Alimentos (CITA), Universidad de Costa Rica, Código Postal 11501-2060 San José,Costa Rica○Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), UMR 95 QUALISUD, 34967
Montpellier Cedex 2, France△Laboratoire de Pharmacologie et Physiopathologie expérimentales, Faculté de Pharmacie, Université Montpellier 1, UMR 95QUALISUD, 34967 Montpellier Cedex 2, France

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Gabriela Azofeifa,*,† Silvia Quesada,† Frederic Boudard,‡ Marion Morena,§,∥ Jean-Paul Cristol,§,∥Ana M. Pérez,⊥ Fabrice Vaillant,○ and Alain Michel△

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