Anthocyanins Delay Ageing-Related Degenerative Changes in the Liver
Abstract
Liver ageing is a significant risk factor for chronic liver diseases. Anthocyanin is a food additive that has previously shown efficacy in increasing longevity. Here, we tested whether anthocyanins could protect young mice from accelerated ageing of the liver. Kunming mice were injected with Dgalactose to accelerate ageing and were given 20 or 40 mg/kg anthocyanins as an intervention. After eight weeks, whole liver function and structure were evaluated, and the expression levels of genes involved in the DNA damage signalling pathway were assessed by Western blot analysis. Anthocyanins delayed the reduction of the liver index (p < 0.05), hepatic tissue injury and fibrosis. Anthocyanins also maintained the stability of the redox system (GSH-PX, T-SOD and MDA) in plasma and liver structures (p < 0.001) and reduced the levels of inflammatory factors (IL-1, IL-6 and TNF-α) in the liver (p < 0.05). Moreover, the expression levels of sensors (ATM and ATR), mediators (H2AX and γ-H2AX) and effectors (Chk1, Chk2, p53 and p-p53) in the DNA damage signalling pathway were all reduced. Anthocyanins could be widely used in the field of health products to slow ageing-related deterioration of liver function and structure by inhibiting DNA damage.
肝脏老化是慢性肝病的重要危险因素。花青素是一种食品添加剂，以前已经显示出延长寿命的功效。在这里，我们测试了花青素是否可以保护幼龄小鼠免于肝脏加速衰老。昆明小鼠注射d半乳糖加速衰老，并给予20或40 mg/kg花青素干预。8周后，评估全肝功能和结构，并通过Western blot分析检测DNA损伤信号通路相关基因的表达水平。花青素延缓肝脏指数的降低(p <0.05)、肝组织损伤及纤维化。花青素还能维持血浆和肝脏结构中氧化还原系统(GSH-PX、T-SOD和MDA)的稳定性(p <降低肝脏炎症因子(IL-1、IL-6和TNF-α)水平(p <０．０５)． 此外，DNA损伤信号通路中传感器(ATM和ATR)、介质(H2AX和γ-H2AX)和效应子(Chk1、Chk2、p53和p-p53)的表达水平均降低。花青素可通过抑制DNA损伤来延缓与衰老相关的肝功能和结构的恶化，可广泛应用于保健品领域。
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