Antiulcer Activity of Anthocyanins from Rubus coreanus via Association with Regulation of the Activity of Matrix Metalloproteinase-2

ABSTRACT:

Anthocyanins were extracted from the fruits of Rubus coreanus. Whether their antioxidant properties and antiulcer activity in gastric ulceration have been accompanied by the activation of matrix metalloproteainse-2 (MMP-2) was investigated. To assess the effect of anthocyanins on gastric ulcer, the rats were administered with anthocyanins (20, 50, and 80 mg/kg of body weight) before treatment with naproxen (80 mg/kg of body weight) to induce gastric ulceration. Lipid peroxidation and the activities of radical scavenging enzymes such as catalase, superoxide dismutase, and glutathione peroxidase were determined. The MMP-2 level was tested by zymography and Western blot. Anthocyanins of R. coreanus exhibit possible antiulcer activity in acute ulcer in a rat model by preventing lipid peroxidation and a significant increase in the activities of antioxidant enzymes such as catalase, superoxide dismutase, and glutathione peroxidase. Also, anthocyanins induce activation of MMP-2 and attenuate the activity of the proinflammatory molecules, such as tumor necrosis factor-α and interleukin-1β.

从悬钩子果实中提取花青素。研究其抗氧化和抗溃疡活性是否伴随着基质金属蛋白酶-2 (MMP-2)的激活。在给予萘普生(80 mg/kg体重)诱导胃溃疡前，分别给予20、50、80 mg/kg体重的花青素，观察花青素对大鼠胃溃疡的影响。测定脂质过氧化及过氧化氢酶、超氧化物歧化酶和谷胱甘肽过氧化物酶等自由基清除酶活性。酶谱和Western blot检测MMP-2水平。在大鼠急性溃疡模型中，金针菇花青素通过抑制脂质过氧化和显著提高过氧化氢酶、超氧化物歧化酶和谷胱甘肽过氧化物酶等抗氧化酶的活性，表现出可能的抗溃疡活性。此外，花青素还能诱导MMP-2的激活，减弱促炎分子的活性，如肿瘤坏死因子-α和白细胞介素-1β。

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http://med.wanfangdata.com.cn/Paper/Detail/PeriodicalPaper_PM21942741