黑莓酒多糖的结构特征及对LPS激活的RAW 264.7巨噬细胞的免疫调节作用

ABSTRACT：Three polysaccharide fractions were isolated from blackberry wine. The crude extract BWPs was obtained with ethanol precipitation and freeze-thawing process, it was then submitted to Fehling treatment, giving soluble BWPFs and insoluble BWPFp fractions. These fractions were characterized by Gas Chromatography-Mass Spectrometry (GC–MS) and Nuclear Magnetic Resonance (NMR). Major polysaccharides were identified for each fraction: mannan, type II arabinogalactan and type I rhamnogalacturonan for BWPs, a mannan formed by a major chain of α-Manp(1→6)-linked units, O-2 substituted with α-D-Manp(1→2)-linked side chains for BWPFp and a AG II formed by a major chain of β-D-Galp(1→3)-linked, substituted at O-6 by side chains of the β-D-Galp(1→6)-linked, which then are substituted at O-3 by non-reducing units of α-L-Araf and a RG I, formed by [→4)-α-D-GalpA-(1→2)-α-L-Rhap-(1→]n for BWPFs. Anti-inflammatory effects of polysaccharide fractions were evaluated in RAW 264.7 cells. Fractions markedly reduced nitric oxide (NO) and pro-inflammatory cytokine production(TNF-α and IL-1β) in LPS-treated cells.

KEY WORDS: Blackberry wine Polysaccharides Anti-inflammatory activity NMR GC–MS

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Structural characterization of blackberry wine polysaccharides and immunomodulatory effects on LPS-activated RAW 264.7 macrophages

Adriana Rute Cordeiro Caillota, Iglesias de Lacerda Bezerraa,Laís Cristina Gusmão Ferreira Palharesb, Arquimedes Paixão Santana-Filhoa,Suely Ferreira Chavanteb, Guilherme Lanzi Sassakia,⁎

a Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Curitiba, Paraná, 81.531-980, Brazil
b Laboratório Glucoconjugados Bioativos, Departamento de Bioquímica, Universidade Federal do Rio Grande do Norte, Natal, Rio Grande do Norte 59.078-970, Brazil

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黑莓药理作用研究进展