黑莓花色苷对过氧化氢诱导血管内皮细胞损伤的保护作用

摘要：目的：研究黑莓花色苷提取物对过氧化氢(H：0：)诱导损伤的血管内皮细胞的保护作用及机理。方法：采用倒置相差显微镜观察凋亡细胞的形态学特征，MTI"法观察细胞存活率，试剂盒法测定抗氧化酶活性，流式细胞计法测定细胞凋亡。结果：用不同剂量的黑莓花色苷预处理，细胞形态与损伤组相比较为完整。经50～200肛g／mL黑莓花色苷预处理，内皮细胞存活率由106．5％升至157．0％，显著降低H：O：对血管内皮细胞增殖的抑制作用(P<0．01)；黑莓花色苷预处理可显著提高血管内皮细胞过氧化物酶(POD)和超氧化物歧化酶(SOD)活性，200pg／mL黑莓花色苷预处理使POD活性提高50％；而血管内皮细胞的早期凋亡率从12．96％降低到5．21％，晚期凋亡从18．16％降低到2．01％，与H：O：处理比，极显著降低(P<0．01)。结论：黑莓花色苷通过提高细胞存活率，提高细胞抗氧化酶活性，抑制细胞凋亡，对H：0：诱导的血管内皮细胞损伤起到保护作用。

关键词：黑莓花色苷；血管内皮细胞；氧化损伤；凋亡

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参考文献：

[1] ARMSTRONG A W，VOYLES S V，ARMSTRONG E J，et a1．Angiogenesis and oxidative stress：common mechanisms linking psoriasis with atherosclerosis[J]．Journal of Dermatological Science，201 1，63(1)：1-9．
[2] GRIENDLING K K，FITZGERALD G A．Oxidative stress andcardiovascular injury Part II：animal and human studies[J]．Circulation，2003，108(17)：2034—2040．
[3]QIN F’LIU YX，ZHAO HW，et a1．Chinese medicinal formula Guan—Xin-Er-Hao protects the heart against oxidative stress induced by acuteischemic myocardial injury in rats[J]．Phytomedicine，2009，16(2)：215．221．
[4]YEN P L，CHEN B H，YANG F L，et a1．Effects of deep-frying oil onblood pressure and oxidative stress in spontaneously hypertensive andnormotensive rats[J]．Nutrition，2010，26(3)：331-336．
[5]YI Long，CHEN Cunye，JIN Xin，et a1．Structural requirements ofanthocyanins in relation to inhibition of endothelial injury inducedby oxidized low—density lipoprotein and correlation with radicalscavenging activity[J]．FEBS Letters，2010，584(3)：583-590．
[6]SHIM S H，KIM J M，CHOI C Y，et a1．Ginkgo biloba extract andbilberry anthocyanins improve visual function in patienKs witll normaltension glaucoma[J]．Journal of Medicinal Food，2012，15(9)：818—823．
[7]HWANG J W．KIM E K，LEE S J，et a1．Antioxidant activity andprotective effect of anthocyanin oligomers on H202-triggered G#Marrest in retinal cells[J]．Journal of Agricultural and Food Chemistry，2012，印(17):4282-4288．
[8]HARDING H P，ZHANG Y，ZENG H，et a1．An integrated stressresponse regulates amino acid metabolism and resistance to oxidativestress[J]．Molecular Cell，2003，1 1(3)：619—633．
[9]GARCIA—ALONSO M，RIMBACH G。RIVAS．GONZALO J C，et a1．Antioxidant and cellular activities of anthocyanins and theircorresponding vitisins a studies in platelets，monocytes，and humanendothelial cells[J] Journal of Agncultural and Food Chemistry，2004，52(1 11：3378—3384．
[10]SARMA A D，SHARMA R．Anthocyanin—DNA copigmentationcomplex：mutual protection against oxidative damage[J】．Phytochemistry，1999，52(7)：1313-1318．
[11]PERGOLA C，ROSSI A，DUGO P，et a1．Inhibition of nitric oxidebiosynthesis by anthocyanin fraction of blackberry extract[J]．NitricOxide，2006，15(1)：30-39．
[12]AMORINI A M，LAZZARINO G，GALVANO F，et a1．Cyanidin．3-O-B—glucopyranoside protects myocardium and erythrocytes fromoxygen radical-mediated damages[J]．Free Radical Research，2003，37(4)：453-460．
[13]JIA Na，XIONG YoulingL．，KONG Baohua’et a1．Radical scavengingactivity of black currant(Ribes nigrum L．)extract and its inhibitoryeffect on gastric cancer cell proliferation via induction of apoptosis[J]．Journal ofFunctional Foods，2012，4(1)：382—390．
[14]YOUDIM K A，MARTIN A。JOSEPH J A．Incorporation of the elderberry anthocyanins by endothelial cells increases protection against oxidative stress[J]．Free Radical Biology and Medicine，2000， 29(1)：51—60．
[15]HEO HJ．LEE C Y．Strawberry and its anthocyanins reduce oxidafive stress—induced apoptosis in PCI2 cells[J]．Journal of Agricultural and Food Chemistry，2005，53(6)：1984．1999．
[16]YE Junli，A任NG Xi删un，YAN Cunling，et a1．Effect of purple sweet potato anthocyanins on fl-amyloid-mediated PC·12 cells death by inhibition ofoxidative saess[J]．Neurochemical Research，2010，35(3)：357-365．
[17]SOLOMON A，GoI工『BOWICZ S．YABLOWICZ Z．et a1．Protection of fibroblasts(NIH一3T3)against oxidative damage by cyanidin-3-rhamncIglucoside isolated from Fig Fruits(凡cus carica L．)【J】．Journal of Agricultural and Food Chemistry，2010，58(1 1)：6660一6665．
[18]QUESADA I M，DEL BAS J M，BLAD C，et a1．Grape seed procyartidins inhibit the expression of metallothione in genes in human HepG2 cells[J]．Genes＆Nutrition，2007，2(1)：105一109．
[19]ZHA0 Xincan，ZHANG Li，YU Hu，et a1．Curcumin protects mouse neuroblastoma Neuro-2A cells against hydrogen·-peroxide·-induced oxidative stress[J]．Food Chemistry，201 1，129(2)：387．394．
[20]ZHU w，JIA Q，WANG Y，et a1．The anthocyanin cyanidin一3一O一glucoside，a flavonoid，increases hepatic glutathione synthesis and protects hepatocytes against reactive oxygen species during hyperglycemia：involvement of a cAMP—PKA-dependent signalingpathway[J]．Free Radical Biology and Medicine，2012，52(2)：314-327．
[21]CHUHL'CHIEN J C．DUH PD．Protective effect of Cordyceps militaris against high glucose—induced oxidative stress in human umbilical vein endothelial cells[J1．Food Chemistry，201 1，129(3)：871．876．