Abstract:The aim of this study was to investigate the protective ability of blackberry extract (BE) against oxidative stress in carbon tetrachloride (CCl4)-treated rats. The results showed that treatment with BE attenuated lipid peroxidation that was increased by CCl4 and also markedly recovered the activity of antioxidant enzymes, such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR), that were decreased by CCl4. BE also elevated the protein expression levels of NF-E2-related factor-2 (Nrf2), CuZnSOD,MnSOD,GPx-1/2, and heme oxygenase-1 (HO-1), but not that of catalase. Furthermore, the administration of BE significantly attenuated the levels of serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) that were increased by CCl4. Therefore, the present study suggests that BE possesses significant protective effects against in vivo oxidative stress.

Key words:blackberry, carbon tetrachloride, oxidative stress, antioxidant enzyme, lipid peroxidation, NF-E2-related factor-2 (Nrf2)

†Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Jeonbuk 580-185, Republic of Korea‡Department of Culinary Nutrition, Woosong University, Daejeon 300-718, Republic of Korea

Byoung Ok Cho,† Hyung Won Ryu,† Chang Hyun Jin,† Dae Seong Choi,† Si Yong Kang,† Dong Sub Kim,†Myung-Woo Byun,‡ and Il Yun Jeong*,†

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Blackberry Extract Attenuates Oxidative Stress through Up-regulation of Nrf2-Dependent Antioxidant Enzymes in Carbon Tetrachloride-Treated Rats